产品概述
白细胞介素18（IL-18）是分子量为18KD的细胞因子，最早发现在中毒性休克中，作为共刺激因子以产生干扰素γ（IFN-γ）。IL-18的生物学功能与IL-12非常相似。IL-18以没有信号肽，分子量为24KD 的前体形式被合成；有活性、成熟的IL-18必须通过切割前体产成。白细胞介素1β转换酶（ICE, caspase-1）在天冬氨酸的p1位置切开IL-18前体，产生具有生物活性的，可以从细胞内分泌成熟的IL-18。文献报道，IL-18主要来源于活化的巨噬细胞，树突状细胞，Kupffer细胞，角质形成细胞，肠上皮细胞，成骨细胞，肾上腺皮质细胞和小鼠间脑等。
产品原理
本实验采用双抗体夹心ELISA法。抗人IL-18单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗人IL-18抗体，标本、标准品中的IL-18会与加入于孔中的生物素化的抗人IL-18抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有IL-18，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值， IL-18浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中IL-18浓度。
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