产品概述
脂联素又被称作Acrp30,AdipoQ,apM1,GBP28, Acrp30是一种分子量为30KD的脂肪细胞补体相关蛋白，基因转录物apM1是在脂肪组织中表达最丰富的转录物， GBP28是脂肪细胞特异性分泌的28KD凝胶蛋白，在维持机体葡萄糖和脂质平衡上有着重要的作用，脂联素结构包括一个胶原样区域组成的N-末端和球状区域组成的C-末端，此区域有一段与补体因子C1q非常类似的序列，此外脂联素还具有增加抗动脉粥样硬化和抗炎症等生物学效应，在机体产生疾病时血浆中的脂联素水平会降低。
产品原理
本实验采用双抗体夹心ELISA法。抗人Adiponectin单抗包被于酶标板上，标本和标准品中的Adiponectin会与单抗结合形成免疫复合物，游离的成分被洗去；加入生物素化的抗人Adiponectin抗体，生物素化抗人Adiponectin抗体与酶标板上结合的标准品或样本中的Adiponectin结合而形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有Adiponectin，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，Adiponectin浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中Adiponectin浓度。
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