Shanghai Excell Biological Technology Co., Ltd.

ResiQuant® Residual Host DNA Sample Pretreatment Kit (Magnetic Bead Method) is used to extract host cell DNA from biopharmaceutical raw materials, finished products, semi-finished products, intermediates product and process monitoring samples produced by different cell lines such as CHO, E.coli, Human, Vero, Pichia, NS0 and MDCK .The pretreatment kit uses chemical lysis and magnetic beads to extract genomic DNA from different types of samples, including samples with high protein and low DNA concentration ,which can effectively extract and purify trace amounts of DNA.
To quantify host residual DNA, it is recommended to use this kit in conjunction with our company's DNA Residue Detection Kit (Fluorescent Probe qPCR Method).

Product Advantages
1.Simple and fast: simple operation and short time consumption;
2.Good compatibility: compatible with other residua DNA detection kits;
3.Magnetic bead extraction: meet high-throughput requirements;
4.High recovery rate：the sample recovery rate reaches 70%-130%, comply with national pharmacopoeia requirements;

Simulated samples		Spike concentration(fg/μL)	Sample detemination mean(fg/μL)	CV(%)	Recovery rate(%)
DLB(NEG)	high	150000	121233	3%	81%
	medium	15000	1379	6%	92%
	low	15	16	10%	105%
Matrix BSA (10ml/mL)	high	150000	145733	3%	97%
	medium	15000	1696	7%	113%
	low	15	18	4%	117%
Matrix BSA (100ml/mL)	high	150000	1119800	11%	80%
	medium	15000	1045	11%	70%
	low	15	13	8%	91%

ResiQuant® Residual Host DNA Sample Pretreatment Kit (Magnetic Bead Method) can extract trace amounts of residual host cell genomic DNA from intermediate products to finished products in the biopharmaceutical production process ,and is suitable for various commonly used host cell types.

ResiQuant® Residual Host DNA Sample Pretreatment Kit（CRB00-0011）

ResiQuant® Residual Host DNA Sample Pretreatment Kit（CRB00-0011）

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