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ResiQuant® Mycoplasma Detection Kit （CRB00-1011）

ResiQuant® Mycoplasma Detection Kit （CRB00-1011）

ResiQuant® Mycoplasma Detection Kit (Fluorescent Probe qPCR Method)

Catalog No.
Size
CRB00-1011
 50T
CRB00-1012
 100T


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ResiQuant® Mycoplasma Detection Kit (Fluorescent Probe qPCR Method) is used in conjunction with ResiQuant® Mycoplasma DNA Extraction And Purification Kit (Magnetic Bead Method)（CAT. CRB00-0031/CRB00-0032）, it can qualitatively detect whether there is mycoplasma contamination in the main cell bank, working cell bank, virus seed batch and cell products.
This kit uses fluorescent probe qPCR technology to design primes probes in the conserved gene fragments of mycoplasma. Based on the sequence comparison results, it can quantitatively detect about 174 species of mycoplasma. The detection is highly specific, and bacteria closely related to mycoplasma (Lactobacillus, Clostridium, Streptococcus, etc.) and common engineering cells such as CHO, VERO, HEK293, etc. are not detected. Verified according to the relevant requirements of EP 2.6.7, JP XVIII and USP <77> for mycoplasma detection, the detection limit can reach 10 CFU/mL，which meets the detection sensitivity requirements of EP 2.6.7 for the NAT method instead of the culture method.
This kit introduces an uracil-N-glycosylase (UNG) anti-contamination system, which can effectively remove residual contamination of PCR products and avoid false positives caused by contamination of amplified products. This kit uses the FAM+HEX/VIC dual-channel detection method and include PC (positive control) and RC (recovery control), which are used to monitor detection sensitively and sample recovery to avoid false negatives. The reagent component contains the reference dye ROX, which is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays the role of fluorescence reference light path correction.
For first-time use, it is recommended to conduct a sample suitability study, including matrix interference, detection limit and specificity. The mycoplasma nucleic acid content in low-level contamination samples（≤10 CFU/mL）does not conform to the normal distribution. This kit uses a 3-well reporting method with a confidence level of more than 90%.

Product Features
1.High Sensitivity: The detection limit is 10CFU/mL, compared with the culture method, the sensitivity is equivalent to or better than the culture method;
2.Wide Coverage: Approximately 174 species of mycoplasma can be detected;
3.False Positive Control: Use touch down qPCR technology to reduce non-specific amplification and background dryness, and introduce the UNG anti-pollution system to reduce contamination caused by amplification products; effectively avoiding false positives;
4.False Negative Control: Use a dual-channel detection method, including PC (positive control) and RC (recovery control), to monitor sample recovery and matrix interference to avoid false negatives;
5.Product Compliance: Verified in accordance with European Pharmacopoeia EP 2.6.7 and Japanese Pharmacopoeia JP G3, and meets regulatory requirements;