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ResiQuant® E.coli HCP ELISA Kit（CRH00-3041）

ResiQuant® E.coli HCP ELISA Kit（CRH00-3041）

ResiQuant® E.coli HCP ELISA Kit (plasmid)

Catalog No.
Size
CRH00-3041
 48T
CRH00-3042
 96T

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Overview performance Related application image Data and files literature Q&A COA

Plasmids are closed-loop, double-stranded, superhelical DNA molecules that are widely used in vaccine preparation and cellular gene therapy (CGT), such as DNA vaccines, naked plasmid gene vectors, adeno-associated viruses (AAVs) and lentiviral vectors, etc. Usually, plasmids are produced in Escherichia coli (E.coli) as the host, and the most representative host bacteria are DH5a, Stbl3, Top10 and other strains derived from E.coli K-12. In the process of plasmid production and purification, there may be contamination of E.coli host cell protein (E.coli HCP) residues, and these residual HCPs may bring risks to the subsequent application of the plasmid, such as generating an immune response in vivo, so the amount of E.coli HCP residues should be controlled. Unlike the conventional process of expressing recombinant proteins using E.coli, the most commonly used process for plasmid production and purification is the alkaline lysis process. The antigen used in this kit is denatured E.coli HCP prepared based on the E.coli alkaline cleavage process, and the exclusive antibody is obtained by immunizing animals with E.coli HCP using the alkaline cleavage process. This product is suitable for quantitative detection of E.coli HCP in plasmid intermediates, stock solutions and final products extracted by the alkaline lysis process.
Before using the kit for the first time, it is recommended that a product suitability study be completed to confirm that there is no interference with the sample matrix and appropriate dilution conditions for the sample assay.

Product Features
1.Quantitative Range: 1.0~250 ng/mL；
2.Sensitivity: The lower limit of detection(LOD) of E.coli HCP is not higher than 0.4 ng/mL, the lower limit of quantification: 1.0 ng/mL；
3.Precision: The coefficient of variation(CV) for sample concentrations within the quantification range is less than 20% both intra-assay and inter-assay；
4.Specificity: No cross-reactivity with host proteins such as CHO, Vero, HEK 293,etc.；

Product Advantages
Complete traceability system for reagent kits
Independent preparation and development of components (calibrators and antibodies),independent control of raw materials, and perfect traceability system of reagent kits;
HCP preparation process is representative
Selection of representative E. coli strains; simulation of actual sample preparation process;
High specificity
Antibody coverage of up to 84%; contains the vast majority of E.coli HCPs (more than 1,000 HCPs in total);
Simple operation and stable results
Two-step design, simple and quick operation, stable results;
Supply chain security
Large batch output, short delivery time, stable supply;