产品概述
白细胞介素-6是在主体防御中起重要作用的多功能细胞因子，包括急性感染期、免疫应答、神经细胞反应和造血作用。IL-6被常规细胞和转化淋巴细胞、非淋巴细胞所表达。IL-6的表达上调被促有丝分裂信号、抗原刺激信号、脂多糖、钙离子、细胞因子和病毒等众多信号控制。IL-4、IL-10和IL-3调控IL-6在单核细胞中的表达。血清中IL-6水平已经应用在大量的病理检测中，这些检测共包括细菌、病毒感染、外伤、免疫系统疾病和恶性肿瘤。大鼠IL-6 cDNA含211氨基酸残基的多肽前体蛋白，其具有一个在成熟蛋白的第187氨基酸残基位点的狂犬病信号肽。在蛋白序列水平上，大鼠IL-6和人的同源性约为39%，和小鼠IL-6的同源性为87%。
产品原理
本实验采用双抗体夹心ELISA法。抗大鼠IL-6单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗大鼠IL-6抗体，标本、标准品中的IL-6会与加入于孔中的生物素化的抗大鼠IL-6抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有IL-6，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值， IL-6浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中IL-6浓度。
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