产品概述
胰岛素是由胰岛β细胞受内源性或外源性物质如葡萄糖、乳糖、核糖、精氨酸、胰高血糖素等的刺激而分泌的一种蛋白质激素。由A、B链组成，共含51个氨基酸残基。能增强细胞对葡萄糖的摄取利用，对蛋白质及脂质代谢有促进合成的作用。胰岛素是机体内唯一降低血糖的激素，也是唯一同时促进糖原、脂肪、蛋白质合成的激素。
产品原理
本实验采用双抗体夹心ELISA法。抗大鼠Insulin单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗大鼠Insulin抗体，标本、标准品中的Insulin会与加入于孔中的生物素化的抗大鼠Insulin抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有Insulin，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，Insulin浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中Insulin浓度。
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