产品概述
成熟的脑源性神经生长因子(BDNF，brain-derivedneurotrophicfactor)，是一个分子量为13KD，由119个氨基酸残基组成的非糖基化多肽。BDNF在氨基酸水平与NGF有52%同源性，在体液中是以非共价连接的同型二聚体。能够表达BDNF的细胞有成纤维细胞，脑星型胶质细胞，神经细胞，巨核细胞，血小板和平滑肌细胞。在神经系统发育过程中，BDNF可诱导神经细胞的分化，成熟，迁移和触突的形成。在成体，BDNF最主要的作用之一是神经保护作用，可有效防止前脑神经细胞遭受化学破坏而有效减少神经细胞的死亡。
产品原理
本实验采用双抗体夹心ELISA法。抗大鼠BDNF单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗大鼠BDNF抗体，标本、标准品中的BDNF会与加入于孔中的生物素化的抗大鼠BDNF抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有BDNF，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，BDNF浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中BDNF浓度。
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