本实验采用双抗体夹心ELISA法。抗小鼠IgE抗体包被于酶标板上，加入标准品和待测标本，标准品和标本中的IgE会与包被于酶标板上的抗体结合，形成免疫复合物，游离的成分被洗去；加入生物素化的抗小鼠IgE抗体和辣根过氧化物酶标记的亲合素，抗小鼠IgE抗体与被捕获的标准品及待测标本结合，生物素与亲和素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有IgE，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，IgE浓度与OD₄₅₀值之间呈正相关，可通过绘制标准曲线求出标本中IgE浓度。

产品性能

1. 灵敏度：最小可测小鼠IgE达2ng/ml。

2.重复性：板内、板间变异系数均<10%。

产品组分及储存条件

试验所需自备仪器和试剂:

1. 酶标仪(450nm检测波长滤光片，570nm或630nm校正波长滤光片)。

2. 高精度加液器及一次性吸头：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒温箱、蒸馏水或去离子水、坐标纸。

• MOUSE IL-5 ELISA KIT（EM019-96）
• MOUSE Insulin ELISA KIT（EM017-96）
• MOUSE IL-13 ELISA KIT（EM016-96）

• ELISA kit 产品手册2019点击下载

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