产品概述
TNF-α，也称为 TNFSFIA，是肿瘤坏死因子家族的成员，在炎症反应与细胞凋亡中发挥重要作用。初始合成时是26kD分子量的233个氨基酸组成II型跨膜蛋白质，包含30个氨基酸组成的胞内区， 26 个氨基酸组成的跨膜区，以及177个氨基酸组成的胞外区。TNF-α胞内通过非共价的连接形成跨膜的同源二聚体。通过 TNF-α 转换酶（TACE）的作用，从跨膜区C端释放出一个由157个氨基酸组成的可溶 TNF-α（s TNF-α 。目前，已知表达TNF-α的人类细胞有：B细胞，结肠柱状上皮细胞，NK细胞， CD3+CD56+肝自然T细胞，巨噬细胞，单核细胞，树突状细胞，CD4+ CD8+ T细胞，肥大细胞，嗜中性粒细胞，角化细胞，血浆细胞以及脂肪细胞等。
产品原理
本实验采用双抗体夹心ELISA法。抗人TNF-α单抗包被于酶标板上，标本和标准品中的TNF-α会与单抗结合形成免疫复合物，游离的成分被洗去；加入生物素化的抗人TNF-α抗体，生物素化抗人TNF-α抗体与酶标板上结合的标准品或样本中的TNF-α结合而形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有TNF-α，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值，TNF-α浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中TNF-α浓度。
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