产品概述
细胞间粘附分子-1（Intercellular adhesion molecule-1，ICAM-1），也叫做CD54，是一个普遍存在的跨膜糖蛋白，在白细胞的迁移和激活中起着非常重要的作用。可溶的ICAM-1被报道出现在血清、脑脊髓液、尿液和支气管肺泡的洗出液中。在这些液体中 sICAM-1（Soluble ICAM-1）量的提高与心血管疾病、II型糖尿病、器官移植失功、氧化剂应激、腹部脂肪聚集、高血压、肝病和一些恶性肿瘤密切相关。sICAM-1促进血管生成并且可以作为血管内皮细胞激活和受损的一个指标。sICAM-1也可以作为跨膜ICAM-1介导活性的抑制剂，比如抑制单核细胞粘附到激活的内皮细胞，抑制肿瘤细胞对NK细胞介导的裂解的敏感性。
产品原理
本实验采用双抗体夹心ELISA法。抗人ICAM-1单抗包被于酶标板上，同时加入标本、标准品和生物素化的抗人ICAM-1抗体，标本、标准品中的ICAM-1会与加入于孔中的生物素化的抗人ICAM-1抗体及包被于酶标板上的单抗结合，形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有ICAM-1，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值， ICAM-1浓度与OD450值之间呈正相关，可通过绘制标准曲线求出标本中ICAM-1浓度。
Zhang ZY et al. MitochondrialDNA-LL-37Complex Promotes Atherosclerosis by Escaping from AutophagicRecognition.Immunity, 2015. Read more (PubMed : 26680206)
Wang H et al. Loss ofYTHDF2-mediatedm6A-dependent mRNA clearance facilitates hematopoietic stem cellregeneration. CellResearch, 2018. Read more (PubMed : 30150673)
Zhang SY et al.Adipocyte-derivedLysophosphatidylcholine Activates Adipocyte and Adipose TissueMacrophage Nod-LikeReceptor Protein 3 Inflammasomes Mediating Homocysteine-InducedInsulinResistance.EBioMedicine, 2018. Read more (Pubmed : 29735414)
He W et al. Vitamin B5 ReducesBacterialGrowth via Regulating Innate Immunity and Adaptive Immunity in MiceInfectedwith Mycobacterium tuberculosis. Front Immunol, 2018. Read more (Pubmed :29535733)
Teng J et al. High-sensitiveimmunosensingof protein biomarker based on interfacial recognition-inducedhomogeneousexponential transcription.Anal Chim Acta, 2019. Read More (PubMed : 31047141)
He MC et al. Paricalcitolalleviateslipopolysaccharide-induced depressive-like behavior by suppressinghypothalamicmicroglia activation and neuroinflammation. Biochem Pharmacol,2019. Read More (Pubmed :30703351)
Su R et al. A NovelImmunosuppressor,(5R)-5-Hydroxytriptolide, Alleviates Movement Disorder andNeuroinflammation ina 6-OHDA Hemiparkinsonian Rat Model. Aging and Disease,2017. Read More (PubMed: 28203480)
