产品概述

单核细胞趋化蛋白1(MCP-1/CCL2)，也称为单核细胞趋化与激活因子（MCAF），是趋化因子家族CC亚族一个成员。MCP-1是一种很有潜能的单核细胞及巨噬细胞的诱生剂及激活因子。人MCP-1 cDNA编码99个氨基酸组成的前体蛋白，当剪切掉23个氨基酸疏水性信号肽后，就形成了由76个氨基酸组成的成熟蛋白。与小鼠趋化因子相比，人MCP-1与小鼠MCP-5（CCL12）、MCP-1/JE(CCL2)最相关，小鼠MCP-5与人MCP-1有65%序列同源性。MCP-1可以被许多炎症介导物诱导，如LPS、 IL-1β、 TNF-α、IFN-γ、IL-6及IL-4等。表达MCP-1的细胞有：单核细胞、肥大细胞、T细胞、成骨细胞、成纤维细胞、内皮细胞、表皮细胞、小胶质细胞、星形胶质细胞及平滑肌细胞等。

产品原理

本实验采用双抗体夹心ELISA法。抗人MCP-1单抗包被于酶标板上，标本和标准品中的MCP-1会与单抗结合形成免疫复合物，游离的成分被洗去；加入生物素化的抗人MCP-1抗体，生物素化抗人MCP-1抗体与酶标板上结合的标准品或样本中的MCP-1结合而形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有MCP-1，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值， MCP-1浓度与OD₄₅₀值之间呈正相关，可通过绘制标准曲线求出标本中MCP-1浓度。

产品性能

1. 灵敏度：最小可测人MCP-1达7pg/ml 。

2. 重复性：板内、板间变异系数均<10%。

产品组分及储存条件

试验所需自备仪器和试剂:

1. 酶标仪(450nm检测波长滤光片，570nm或630nm校正波长滤光片)。

2. 高精度加液器及一次性吸头：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒温箱、蒸馏水或去离子水、坐标纸。

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