本实验采用双抗体夹心ELISA法。抗人DKK-1单抗包被于酶标板上，标本和标准品中的DKK-1会与单抗结合形成免疫复合物，游离的成分被洗去；加入生物素化的抗人DKK-1抗体，生物素化抗人DKK-1抗体与酶标板上结合的标准品或样本中的DKK-1结合而形成免疫复合物，游离的成分被洗去；加入辣根过氧化物酶标记的亲合素，亲合素与生物素特异性结合，游离的成分被洗去。加入显色底物（显色剂），若反应孔中有DKK-1，辣根过氧化物酶会使无色的显色剂变蓝，加终止液变黄。在450nm处测OD值， DKK-1浓度与OD₄₅₀值之间呈正相关，可通过绘制标准曲线求出标本中DKK-1浓度。

产品性能

1. 灵敏度：最小可测人DKK-1达30pg/ml 。

2. 重复性：板内、板间变异系数均<10%。

产品组分及储存条件

试验所需自备仪器和试剂:

1. 酶标仪(450nm检测波长滤光片，570nm或630nm校正波长滤光片)。

2. 高精度加液器及一次性吸头：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒温箱、蒸馏水或去离子水、坐标纸。

• HUMAN IL-3 ELISA KIT（EH058-96）
• HUMAN IL-15 ELISA KIT（EH057-96）
• HUMAN MIP-1α ELISA KIT（EH055-96）

• ELISA kit 产品手册2019点击下载

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